Shedding Light on the Shadows: The Untapped Potential of the Dark Kinome

Shedding Light on the Shadows: The Untapped Potential of the Dark Kinome The dark kinome comprises a significant portion of human kinases with poorly understood functions. These understudied kinases represent a rich source of potential novel drug targets. Challenges in studying dark kinases include low expression and difficult-to-characterize catalytic domains. Technological advancements are now enabling […]

Revolutionizing Protein Structure Analysis: Cryo-EM without Purification Accelerates the Future of Structural Biology

Revolutionizing Protein Structure Analysis: Cryo-EM without Purification Accelerates the Future of Structural Biology New workflow enables solving protein structures via cryo-EM directly from crude cell-free lysates with no or minimal purification. The PURE expression system provides minimal background, supporting atomic-resolution reconstructions in under 24 hours. Miniaturized 25µl reactions are sufficient, reducing costs and labor. Additional […]

Multi-Step LLM Prompt: From Protein Sequence to Validated Protein Structure

From Sequence to Structure—How LLMs Unlock Protein Mysteries LLMs enable multi-step workflows from raw protein sequence to validated 3D model, combining language and structure understanding. Secondary, tertiary structure prediction and validation can all be orchestrated through evolving AI prompts and specialized models. Key validation metrics include Ramachandran plots, MolProbity scores, and checks for stereochemical plausibility. […]

Supercharging Discovery: How LLM Prompts Are Transforming Structural Biology and Cheminformatics

Supercharging Discovery: How LLM Prompts Are Transforming Structural Biology and Cheminformatics Motif/fingerprint discovery from single protein sequences. Prediction of protein–protein interactions from sequence pairs. Mapping from sequence to secondary structure and risk features. Analog design suggestions for improved drug properties. Pathway inference using protein functional annotations. Rapid metabolic/toxicity profiling and mitigation strategies. Patent mining and […]